3 Minute 3Rs August 2020

You’re listening to the August episode of 3 Minute 3Rs.

The papers behind the pod:

1.      The ARRIVE guidelines 2.0: Updated guidelines for reporting animal research. PloS Biology https://journals.plos.org/plosbiology/article?id=10.1371/journal.pbio.3000410

2.      Acute and chronic stress prevents responses to pain in zebrafish: evidence for stress-induced analgesia. Journal of Experimental Biology. https://jeb.biologists.org/content/223/14/jeb224527

3.      3Rs-based optimization of mice behavioral testing: The habituation/dishabituation olfactory test. Journal of Neuroscience Methods. https://doi.org/10.1016/j.jneumeth.2019.108550



It’s the 3rd Thursday of August, and you’re listening to 3 Minute 3Rs, your monthly recap of efforts to replace, reduce, and refine the use of animals in research. This month we’ve got papers on fish pain and a refined mouse olfaction task. But let’s kick the episode off with the arrival of ARRIVE 2.0

[NC3Rs] Improving the reproducibility of biomedical research is a major challenge. A lack of experimental detail in publications reporting in vivo experiments, such as experimental design and statistical analysis used, means researchers cannot adequately scrutinise the experimental results or repeat experiments. Consequently, data from these published experiments do not contribute to the knowledge base as researchers need to perform or to develop experimental procedures themselves. To highlight the information required the ARRIVE guidelines were developed in 2010. Although these guidelines were widely endorsed by journals and funders they have not led to improvements in reporting.

To address this, ARRIVE 2.0 has been launched and published in PLOS Biology. The recommendations have been organised into two sets, so scientists can more easily incorporate them into their reporting. The first set, the essential 10 constitute the minimum reporting requirement for reviewers and readers to assess the reliability of the findings presented. The second set, the recommended set, provides further context to the study described. An Explanation and Elaboration document giving further information about each of the 21 items in ARRIVE 2.0 has also been published in PLOS Biology.

To learn more about ARRIVE 2.0, explore the recommendations and find resources, visit its new home on the web at arriveguidelines.org.


[Lab Animal] Next up, let’s think about pain processing. In mammals, studies have shown that stress can alter pain-detection thresholds. Acute stress can have an analgesic effect, while chronic sources can enhance responses to pain, an effect known as hyperalgesia. Both can potentially confound experiments.

Scientists at the University of Liverpool recently explored whether such stress-induced responses are also evident in zebrafish.

The short answer? Yes. The team looked at the behavior of acutely and chronically stressed fish, as well as stress-free controls, after fin clipping. Following the potentially painful procedure, unstressed fish swam more slowly and lingered near the bottom of their tanks.

No such change in behavior was observed in...

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